Ancient diversity of splicing motifs and protein surfaces in the wild emmer wheat (Triticum dicoccoides) LR10 coiled coil (CC) and leucine-rich repeat (LRR) domain

Sela, H., Spiridon, L. N., Petrescu, A. J., Akerman, M., Mandel-Gutfreund, Y., Nevo, E., Loutre, C., Keller, B., Schulman, A. H., Fahima, T. (April 2012) Ancient diversity of splicing motifs and protein surfaces in the wild emmer wheat (Triticum dicoccoides) LR10 coiled coil (CC) and leucine-rich repeat (LRR) domain. Molecular Plant Pathology, 13 (3). pp. 276-287. ISSN 14646722 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/21952112
DOI: 10.1111/j.1364-3703.2011.00744.x

Abstract

In this study, we explore the diversity and its distribution along the wheat leaf rust resistance protein LR10 three-dimensional structure. Lr10 is a leaf rust resistance gene encoding a coiled coil-nucleotide-binding site-leucine-rich repeat (CC-NBS-LRR) class of protein. Lr10 was cloned and sequenced from 58 accessions representing diverse habitats of wild emmer wheat in Israel. Nucleotide diversity was very high relative to other wild emmer wheat genes (π= 0.029). The CC domain was found to be the most diverse domain and subject to positive selection. Superimposition of the diversity on the CC three-dimensional structure showed that some of the variable and positively selected residues were solvent exposed and may interact with other proteins. The LRR domain was relatively conserved, but showed a hotspot of amino acid variation between two haplotypes in the ninth repeat. This repeat was longer than the other LRRs, and three-dimensional modelling suggested that an extensive α helix structure was formed in this region. The two haplotypes also differed in splicing regulation motifs. In genotypes with one haplotype, an intron was alternatively spliced in this region, whereas, in genotypes with the other haplotype, this intron did not splice at all. The two haplotypes are proposed to be ancient and maintained by balancing selection. © 2011 The Authors. Molecular Plant Pathology © 2011 BSPP and Blackwell Publishing Ltd.

Item Type: Paper
Uncontrolled Keywords: Puccinia triticina Triticum aestivum Triticum turgidum subsp. dicoccoides
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor
CSHL Authors:
Communities: CSHL labs > Krainer lab
CSHL Post Doctoral Fellows
Depositing User: Matt Covey
Date: April 2012
Date Deposited: 22 Jan 2013 19:31
Last Modified: 01 May 2013 19:34
Related URLs:
URI: https://repository.cshl.edu/id/eprint/27041

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