Fragile X-related protein and VIG associate with the RNA interference machinery

Caudy, A. A., Myers, M., Hannon, G. J., Hammond, S. M. (2002) Fragile X-related protein and VIG associate with the RNA interference machinery. Genes and Development, 16 (19). pp. 2491-2496. ISSN 08909369 (ISSN)

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Abstract

RNA interference (RNAi) is a flexible gene silencing mechanism that responds to double-stranded RNA by suppressing homologous genes. Here, we report the characterization of RNAi effector complexes (RISCs) that contain small interfering RNAs and microRNAs (miRNAs). We identify two putative RNA-binding proteins, the Drosophila homolog of the fragile X mental retardation protein (FMRP), dFXR, and VIG (Vasa intronic gene), through their association with RISC. FMRP, the product of the human fragile X locus, regulates the expression of numerous mRNAs via an unknown mechanism. The possibility that dFXR, and potentially FMRP, use, at least in part, an RNAi-related mechanism for target recognition suggests a potentially important link between RNAi and human disease.

Item Type:	Paper
Uncontrolled Keywords:	Ago-1 Ago-2, miRNA dFXR RNA interference VIG fragile X mental retardation protein messenger RNA protein RNA RNA binding protein unclassified drug vasa intornic protein article Drosophila fragile X syndrome gene expression regulation gene locus gene silencing human nonhuman priority journal regulatory mechanism RNA processing sequence homology Amino Acid Sequence Animals Cell Line Drosophila melanogaster Drosophila Proteins Insect Proteins MicroRNAs Molecular Sequence Data RNA, Double-Stranded RNA, Small Interfering RNA-Binding Proteins RNA-Induced Silencing Complex Arachnida Hexapoda
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi diseases & disorders > congenital hereditary genetic diseases > fragile X syndrome
CSHL Authors:	Caudy, Amy A. Hammond, Scott M. Hannon, Gregory J. Myers, Michael, P
Communities:	CSHL labs > Hannon lab School of Biological Sciences > Publications
Depositing User:	Matt Covey
Date:	2002
Date Deposited:	09 Jan 2013 16:55
Last Modified:	19 Sep 2014 13:55
PMCID:	PMC187452
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/26470

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