Intron-dependent recruitment of pre-mRNA splicing factors to sites of transcription

Huang, S., Spector, D. L. (1996) Intron-dependent recruitment of pre-mRNA splicing factors to sites of transcription. Journal of Cell Biology, 133 (4). pp. 719-732. ISSN 0021-9525

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Abstract

We have examined the nuclear localization of transiently and stably expressed nascent RNA transcripts containing or lacking introns in order to determine if the spatial association of RNA transcripts and pre-mRNA splicing factors in nuclei is random or functionally significant. Our findings show that the association between nascent RNA and splicing factors in the nucleus is intron-dependent when the RNAs are either transiently or stably expressed. Furthermore, our data indicate that splicing factors are recruited to the transcription sites. The presence of both pre-and mRNA at these locations suggest that pre-mRNA splicing occurs at these sites of transcription. In addition, electron microscopic examination of the highly active transcription sites has revealed a granular appearance which closely resembles, but is functionally different from, interchromatin granule clusters. Our findings demonstrate that the nucleus is highly organized and dynamic with regard to the functions of the transcription and pre-mRNA splicing.

Item Type:	Paper
Uncontrolled Keywords:	Cell Nucleus Gene Expression Globins HeLa Cells Humans Introns RNA Precursors RNA Splicing RNA, Messenger RNA-Binding Proteins Templates, Genetic Transcription, Genetic Transfection beta-Galactosidase
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor
CSHL Authors:	Spector, David L.
Communities:	CSHL labs > Spector lab
Depositing User:	Matt Covey
Date:	1996
Date Deposited:	10 Dec 2012 19:43
Last Modified:	29 Jan 2015 19:28
PMCID:	PMC2120843
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/26331

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