In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism

Jimenez-Garcia, L. F., Spector, D. L. (1993) In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism. Cell, 73 (1). pp. 47-59. ISSN 0092-8674

Abstract

We describe the nuclear organization of pre-mRNA processing components in HeLa cells upon adenovirus 2 infection and their relationship to the localization of viral RNA sequences. We observe a redistribution of cellular splicing factors as well as RNA polymerase II and heterogeneous nuclear ribonucleoprotein particle proteins to sites of viral RNA transcription. Similar results were obtained in cells transiently transfected with a plasmid containing a portion of the beta-tropomyosin gene. Our findings demonstrate a very close association between RNA transcripts and transcription and pre-mRNA splicing factors, suggesting that these processes are both temporally and spatially linked in the cell nucleus. Furthermore, these data suggest a recruiting mechanism that regulates the localization of transcription and splicing factors in response to the initiation of active transcription.

Item Type:	Paper
Uncontrolled Keywords:	Adenoviruses, Human Biological Transport Cell Nucleus HeLa Cells Humans Microscopy, Fluorescence Nuclear Proteins RNA Precursors RNA Splicing RNA, Viral Ribonucleoproteins Transcription, Genetic
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor
CSHL Authors:	Spector, David L.
Communities:	CSHL labs > Spector lab
Depositing User:	Matt Covey
Date:	1993
Date Deposited:	10 Dec 2012 19:55
Last Modified:	29 Jan 2015 19:34
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/26323

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