Tani, T., Derby, R. J., Hiraoka, Y., Spector, D. L. (November 1995) Nucleolar accumulation of poly (A)+ RNA in heat-shocked yeast cells: implication of nucleolar involvement in mRNA transport. Molecular Biology of the Cell, 6 (11). pp. 1515-34. ISSN 1059-1524
Abstract
Transport of mRNA from the nucleus to the cytoplasm plays an important role in gene expression in eukaryotic cells. In wild-type Schizosaccharomyces pombe cells poly(A)+ RNA is uniformly distributed throughout the nucleoplasm and cytoplasm. However, we found that a severe heat shock blocks mRNA transport in S. pombe, resulting in the accumulation of bulk poly(A)+ RNA, as well as a specific intron-less transcript, in the nucleoli. Pretreatment of cells with a mild heat shock, which induces heat shock proteins, before a severe heat shock protects the mRNA transport machinery and allows mRNA transport to proceed unimpeded. In heat-shocked S. pombe cells, the nucleolar region condensed into a few compact structures. Interestingly, poly(A)+ RNA accumulated predominantly in the condensed nucleolar regions of the heat-shocked cells. These data suggest that the yeast nucleolus may play a role in mRNA transport in addition to its roles in rRNA synthesis and preribosome assembly.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Sequence Cell Nucleolus drug effects metabolism ultrastructure Cycloheximide pharmacology Heat In Situ Hybridization Microscopy Electron Molecular Sequence Data Oligonucleotide Probes RNA Messenger metabolism Schizosaccharomyces metabolism ultrastructure |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics |
| CSHL Authors: | |
| Communities: | CSHL labs > Spector lab |
| Depositing User: | Brian Soldo |
| Date: | November 1995 |
| Date Deposited: | 30 Mar 2012 22:41 |
| Last Modified: | 29 Jan 2015 19:30 |
| PMCID: | PMC301308 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25770 |
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