Wright, N. J., Zhong, Y. (January 1998) Serotonin-sensitive leakage channel in Drosophila central neurons. J Neurobiol, 34 (1). pp. 83-95. ISSN 0022-3034 (Print)
Abstract
This article reports the analysis of a novel serotonin (5-HT)-sensitive leak channel. The 5-HT responses were recorded in acutely dissociated Drosophila adult and larval central nervous system (CNS) neurons by the patch-clamp method, in an attempt to establish a model preparation suitable for the genetic study of signal transduction underlying central neurotransmission. Focal perfusion or iontophoresis of 5-HT onto some patched neurons induced either an apparent inward or outward current. This apparent outward current is able to cause a strong hyperpolarization of the neuron. This article focuses on the predominant hyperpolarizing response, which is observed in a significant fraction of larger CNS neurons and in different developmental stages. The hyperpolarizing response is in fact mediated by inhibiting an inward leak current, which has a reversal potential around 0 mV. This 5-HT-sensitive leak current appears to be mediated mainly by one type of newly identified leak channel with a similar reversal potential of 0 mV and a conductance of 24 pS. In addition, it was also demonstrated that neurotransmitter-induced responses in both larval and adult Drosophila CNS neurons can be analyzed in this acutely dissociated preparation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Brain cytology physiology Cells, Cultured Drosophila physiology Ganglia Invertebrate cytology physiology Ion Channels physiology Larva physiology Membrane Potentials physiology Neurons physiology Patch-Clamp Techniques Serotonin physiology |
| Subjects: | organism description > animal > insect > Drosophila Investigative techniques and equipment > patch-clamp recording Investigative techniques and equipment > recording devices > patch-clamp recording organs, tissues, organelles, cell types and functions > tissues types and functions > signal transduction |
| CSHL Authors: | |
| Communities: | CSHL labs > Zhong lab |
| Depositing User: | CSHL Librarian |
| Date: | January 1998 |
| Date Deposited: | 29 Mar 2012 20:44 |
| Last Modified: | 29 Mar 2012 20:44 |
| URI: | https://repository.cshl.edu/id/eprint/25580 |
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