PCNA connects DNA replication to epigenetic inheritance in yeast

Zhang, Z. G., Shibahara, K., Stillman, B. (November 2000) PCNA connects DNA replication to epigenetic inheritance in yeast. Nature, 408 (6809). pp. 221-225. ISSN 0028-0836

URL: http://www.ncbi.nlm.nih.gov/pubmed/11089978
DOI: 10.1038/35041601


Formation of a heterochromatin-like structure results in transcriptional silencing at the HM mating-type loci and telomeres in Saccharomyces cerevisiae(1-3). Once formed, such epigenetically determined structures are inherited for many mitotic divisions(4). Here we show that mutations in the proliferating cell nuclear antigen (PCNA), an essential component at the DNA replication fork(5), reduced repression of genes near a telomere and at the silent mating-type locus, HMR. The pol30-8 mutant displayed coexistence of both repressed (pink) and de-repressed (white) cells within a single colony when assayed with the ADE2 gene inserted at HMR. Unlike pol30-8, the pol30-6 and pol30-79 mutants partially reduced gene silencing at telomeres and the HMR and synergistically decreased silencing in cells lacking chromatin assembly factor 1 (CAF-1). All silencing defective mutants showed reduced binding to CAF-1 in vitro and altered chromatin association of the CAF-1 large subunit in vivo. Thus, PCNA participates in inheritance of both DNA and epigenetic chromatin structures during the S phase of the cell cycle, the latter by at least two mechanisms.

Item Type: Paper
Uncontrolled Keywords: ASSEMBLY FACTOR-I assembly factor 1 ORIGIN RECOGNITION COMPLEX origin recognition complex SACCHAROMYCES-CEREVISIAE Saccharomyces Cereviciae TRANSCRIPTIONAL STATES transcriptional states POLYMERASE-EPSILON polymerase epsilon CELL-CYCLE cell cycle CHROMATIN chromatin REPRESSION repression MUTATIONS mutations PROTEINS proteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: November 2000
Date Deposited: 17 Feb 2012 15:04
Last Modified: 20 Jun 2017 18:51
Related URLs:
URI: https://repository.cshl.edu/id/eprint/25149

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