Yeast autonomously replicating sequence binding factor is involved in nucleotide excision repair

Reed, S. H., Akiyama, M., Stillman, B., Friedberg, E. C. (December 1999) Yeast autonomously replicating sequence binding factor is involved in nucleotide excision repair. Genes and Development, 13 (23). pp. 3052-3058. ISSN 08909369 (ISSN)

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DOI: 10.1101/gad.13.23.3052


Nucleotide excision repair (NER) in yeast is effected by the concerted action of a large complex of proteins. Recently, we identified a stable subcomplex containing the yeast Rad7 and Rad16 proteins. Here, we report the identification of autonomously replicating sequence binding factor 1 (ABF1) as a component of the Rad7/Rad16 NER subcomplex. Yeast ABF1 protein is encoded by an essential gene required for DNA replication, transcriptional regulation, and gene silencing. We show that ABF1 plays a direct role in NER in vitro. Additionally, consistent with a role of ABF1 protein in NER in vivo, we show that certain temperature-sensitive abf1 mutant strains that are defective in DNA replication are specifically defective in the removal of photoproducts by NER and are sensitive to killing by ultraviolet (UV) radiation. These studies define a novel and unexpected role for ABF1 protein during NER in yeast.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: 1 December 1999
Date Deposited: 02 Mar 2012 18:43
Last Modified: 20 Jun 2017 19:22
PMCID: PMC317179
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