Prasanth, S. G., Prasanth, K. V. , Stillman, B. (August 2002) Orc6 involved in DNA replication, chromosome segregation, and cytokinesis. Science, 297 (5583). pp. 1026-31. ISSN 1095-9203 (Electronic)
Abstract
Origin recognition complex (ORC) proteins serve as a landing pad for the assembly of a multiprotein prereplicative complex, which is required to initiate DNA replication. During mitosis, the smallest subunit of human ORC, Orc6, localizes to kinetochores and to a reticular-like structure around the cell periphery. As chromosomes segregate during anaphase, the reticular structures align along the plane of cell division and some Orc6 localizes to the midbody before cells separate. Silencing of Orc6 expression by small interfering RNA (siRNA) resulted in cells with multipolar spindles, aberrant mitosis, formation of multinucleated cells, and decreased DNA replication. Prolonged periods of Orc6 depletion caused a decrease in cell proliferation and increased cell death. These results implicate Orc6 as an essential gene that coordinates chromosome replication and segregation with cytokinesis.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Bromodeoxyuridine metabolism Cell Death Cell Division Cell Line Cell Nucleus metabolism Cells, Cultured Centromere metabolism Chromosome Segregation Chromosomes Human metabolism DNA Replication DNA-Binding Proteins genetics metabolism physiology Fluorescent Antibody Technique Gene Silencing Humans Kinetochores metabolism Mitosis Mitotic Spindle Apparatus ultrastructure Origin Recognition Complex Phenotype Polyploidy RNA Small Interfering RNA Untranslated metabolism pharmacology Recombinant Fusion Proteins analysis Saccharomyces cerevisiae Proteins Transfection Tumor Cells Cultured |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > origin recognition complex |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | 9 August 2002 |
| Date Deposited: | 02 Mar 2012 19:26 |
| Last Modified: | 20 Jun 2017 18:20 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25038 |
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