Cloning, Expression, and Chromosomal Localization of the 140-Kilodalton Subunit of Replication Factor-C from Mice and Humans

Luckow, B., Bunz, F., Stillman, B., Lichter, P., Schutz, G. (March 1994) Cloning, Expression, and Chromosomal Localization of the 140-Kilodalton Subunit of Replication Factor-C from Mice and Humans. Molecular and Cellular Biology, 14 (3). pp. 1626-1634. ISSN 0270-7306

DOI: 10.1128/MCB.14.3.1626


We have isolated a full-length mouse cDNA encoding a lysine-rich protein of 1,131 amino acids with a calculated molecular mass of 126 kDa. The protein binds in a sequence-unspecific manner to DNA, is localized exclusively in the nucleus, and contains a putative ATP binding site and a stretch of 80 amino acids with homology to the carboxy terminus of prokaryotic DNA ligases. On the basis of the following facts, we conclude that the isolated cDNA encodes the 140-kDa subunit of mouse replication factor C (mRFC140). (i) The sequence around the ATP binding site shows significant homology to three small subunits of human replication factor C. (ii) Polyclonal antibodies raised against the protein encoded by this cDNA cross-react with the 140-kDa subunit of purified human replication factor C (hRFC140) and recognize in mouse cell extracts an authentic protein with an apparent molecular mass of 130 kDa. (iii) Sequence comparison with a human cDNA isolated by using tryptic peptide sequence information from purified hRFC140 revealed 83% identity of the encoded proteins. The mRFC140 gene is ubiquitously expressed, and two mRNAs approximately 5.0 and 4.5 kb long have been detected. The gene was mapped by in situ hybridization to mouse chromosome 5, and its human homolog was mapped to chromosome 4 (p13-p14).

Item Type: Paper
Uncontrolled Keywords: VIRUS-40 DNA-REPLICATION virus-40 DNA replication CELL NUCLEAR ANTIGEN cell nuclear antigen ESCHERICHIA-COLI Escherichia Coli SACCHAROMYCES-CEREVISIAE Saccharomyces Cerevisiae NUCLEOTIDE-SEQUENCE nucleotide sequence TARGETING SEQUENCE targeting sequence ACCESSORY PROTEINS accessory proteins POLYMERASE-DELTA polymerase delta HELA-CELLS HELA cells RF-C
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: March 1994
Date Deposited: 06 Mar 2012 15:19
Last Modified: 20 Jun 2017 19:51
PMCID: PMC358521
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