Conserved initiator proteins in eukaryotes

Gavin, K. A., Hidaka, M., Stillman, B. (December 1995) Conserved initiator proteins in eukaryotes. Science, 270 (5242). pp. 1667-71. ISSN 0036-8075 (Print) (Public Dataset)



The origin recognition complex (ORC), a multisubunit protein identified in Saccharomyces cerevisiae, binds to chromosomal replicators and is required for the initiation of cellular DNA replication. Complementary DNAs (cDNAs) encoding proteins related to the two largest subunits of ORC were cloned from various eukaryotes. The cDNAs encoding proteins related to S. cerevisiae Orc1p were cloned from the budding yeast Kluyveromyces lactis, the fission yeast Schizosaccharomyces pombe, and human cells. These proteins show similarity to regulators of the S and M phases of the cell cycle. Genetic analysis of orc1+ from S. pombe reveals that it is essential for cell viability. The cDNAs encoding proteins related to S. cerevisiae Orc2p were cloned from Arabidopsis thaliana, Caenorhabditis elegans, and human cells. The human ORC-related proteins interact in vivo to form a complex. These studies studies suggest that ORC subunits are conserved and that the role of ORC is a general feature of eukaryotic DNA replication.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Arabidopsis chemistry genetics Base Sequence Caenorhabditis elegans chemistry genetics Cloning Molecular Conserved Sequence DNA Replication DNA, Complementary DNA-Binding Proteins chemistry genetics Humans Kluyveromyces chemistry genetics Molecular Sequence Data Origin Recognition Complex Polymerase Chain Reaction Replication Origin Repressor Proteins chemistry genetics Saccharomyces cerevisiae chemistry genetics Saccharomyces cerevisiae Proteins Schizosaccharomyces chemistry genetics growth & development
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > origin recognition complex
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: 8 December 1995
Date Deposited: 06 Mar 2012 17:13
Last Modified: 08 Sep 2017 21:05
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