cDNAs encoding the large subunit of human replication factor C

Bunz, F., Kobayashi, R., Stillman, B. (December 1993) cDNAs encoding the large subunit of human replication factor C. Proc Natl Acad Sci U S A, 90 (23). pp. 11014-8. ISSN 0027-8424 (Print)

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Replication factor C (RFC) is a multisubunit, DNA polymerase accessory protein required for the coordinated synthesis of both DNA strands during simian virus 40 DNA replication in vitro. Previous studies have shown that RFC is a DNA-dependent ATPase that binds in a structure-specific manner to the 3' end of a primer hybridized to a template DNA, an activity thought intrinsic to the 140-kDa component of this multisubunit complex. Here, the isolation and analysis of cDNAs encoding this subunit is described. Analysis of the full-length coding sequence revealed an open reading frame of 3.4 kb, encoding an 1148-amino acid protein with a predicted molecular mass of 130 kDa. A putative ATP-binding motif was observed that is similar to a motif in several of the smaller subunits of RFC and in functionally homologous replication factors of bacterial and viral origin. A "DEAD" box is also conserved among these proteins. The predicted protein shows significant identity with a DNA-binding protein of murine origin (B. Luckow, P. Lichter, and G. Schutz, personal communication). Regions of similarity were also seen between the amino acid sequences of the 140-kDa subunit of RFC, poly(ADP-ribose) polymerase, and bacterial DNA ligases--possibly representing a conserved structural feature of these proteins that bind similar DNA substrates.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Antibodies Monoclonal immunology Base Sequence Cell Line Cloning Molecular Comparative Study DNA Ligases chemistry DNA Primers chemistry DNA Replication DNA, Complementary genetics DNA-Binding Proteins genetics Homeodomain Proteins Humans Mice Molecular Sequence Data Proto-Oncogene Proteins c-bcl-2 Replication Factor C Repressor Proteins Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Homology Amino Acid
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: 1 December 1993
Date Deposited: 07 Mar 2012 18:32
Last Modified: 09 Nov 2017 19:41
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