Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication

Brill, S. J., Stillman, B. (November 1989) Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature, 342 (6245). pp. 92-5. ISSN 0028-0836

DOI: 10.1038/342092a0


Cell-free replication systems for simian virus 40 (SV40) DNA are taken to be a model for the replication of eukaryotic chromosomes, because only one viral protein is required to supplement the replication proteins provided by a human cell extract. To prove that these cellular proteins function in chromosomal DNA replication we have begun to identify homologous proteins in an organism that can be genetically manipulated. Here we report the identification of yeast replication factor-A (yRF-A) from Saccharomyces cerevisiae and show that it is functionally and structurally related to a human protein that is required for the initiation and elongation of SV40 DNA replication. Yeast RF-A, a multi-subunit phosphoprotein, is similar to the human protein in its chromatographic behaviour, subunit structure and DNA-binding activity. The yeast protein will fully substitute for the human protein in an early stage of the initiation of SV40 DNA replication. Substitution of yRF-A in the complete SV40 replication system, however, results in reduced DNA replication, presumably due to a requirement for species-specific interactions between yeast RF-A and the DNA polymerase complex.

Item Type: Paper
Uncontrolled Keywords: Binding Competitive DNA Replication DNA Bacterial metabolism DNA Binding Proteins metabolism Kinetics Molecular Weight Phosphoproteins metabolism Plasmids Saccharomyces cerevisiae metabolism Simian virus 40 genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: 2 November 1989
Date Deposited: 07 Mar 2012 18:20
Last Modified: 20 Jun 2017 20:18
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