Interferon regulatory factors are transcriptional regulators of adipogenesis

Eguchi, J., Yan, Q. W., Schones, D. E., Karnal, M., Hsu, C. H., Zhang, M. Q., Crawford, G. E., Rosen, E. D. (2008) Interferon regulatory factors are transcriptional regulators of adipogenesis. Cell Metabolism, 7 (1). pp. 86-94. ISSN 1550-4131

URL: https://www.ncbi.nlm.nih.gov/pubmed/18177728
DOI: 10.1016/j.cmet.2007.11.002

Abstract

We have sought to identify transcriptional pathways in adipogenesis using an integrated experimental and computational approach. Here, we employ high-throughput DNase hypersensitivity analysis to find regions of altered chromatin structure surrounding key adipocyte genes. Regions that display differentiation-dependent changes in hypersensitivity were used to predict binding sites for proteins involved in adipogenesis. A high-scoring example was a binding motif for interferon regulatory factor (IRF) family members. Expression of all nine mammalian IRF mRNAs is regulated during adipogenesis, and several bind to the identified motifs in a differentiation-dependent manner. Furthermore, several IRF proteins repress differentiation. This analysis suggests an important role for IRF proteins in adipocyte biology and demonstrates the utility of this approach in identifying cis- and trans-acting factors not previously suspected to participate in adipogenesis.

Item Type: Paper
Uncontrolled Keywords: DNASE-HYPERSENSITIVE SITES ADIPOCYTE DIFFERENTIATION ACTIVE CHROMATIN GENE-EXPRESSION CHIP IDENTIFICATION SENSITIVITY MICROARRAYS PROMOTERS PROTEINS
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > analysis and processing > NETBAG
bioinformatics > quantitative biology
CSHL Authors:
Communities: CSHL labs > Zhang lab
Depositing User: Tom Adams
Date: 2008
Date Deposited: 25 Aug 2011 15:50
Last Modified: 13 Mar 2018 20:42
PMCID: PMC2278019
Related URLs:
URI: http://repository.cshl.edu/id/eprint/7722

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