Eguchi, J., Yan, Q. W., Schones, D. E., Karnal, M., Hsu, C. H., Zhang, M. Q., Crawford, G. E., Rosen, E. D. (2008) Interferon regulatory factors are transcriptional regulators of adipogenesis. Cell Metabolism, 7 (1). pp. 86-94. ISSN 1550-4131
Abstract
We have sought to identify transcriptional pathways in adipogenesis using an integrated experimental and computational approach. Here, we employ high-throughput DNase hypersensitivity analysis to find regions of altered chromatin structure surrounding key adipocyte genes. Regions that display differentiation-dependent changes in hypersensitivity were used to predict binding sites for proteins involved in adipogenesis. A high-scoring example was a binding motif for interferon regulatory factor (IRF) family members. Expression of all nine mammalian IRF mRNAs is regulated during adipogenesis, and several bind to the identified motifs in a differentiation-dependent manner. Furthermore, several IRF proteins repress differentiation. This analysis suggests an important role for IRF proteins in adipocyte biology and demonstrates the utility of this approach in identifying cis- and trans-acting factors not previously suspected to participate in adipogenesis.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | DNASE-HYPERSENSITIVE SITES ADIPOCYTE DIFFERENTIATION ACTIVE CHROMATIN GENE-EXPRESSION CHIP IDENTIFICATION SENSITIVITY MICROARRAYS PROMOTERS PROTEINS |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > analysis and processing > NETBAG bioinformatics > quantitative biology |
| CSHL Authors: | |
| Communities: | CSHL labs > Zhang lab |
| Depositing User: | Tom Adams |
| Date: | 2008 |
| Date Deposited: | 25 Aug 2011 15:50 |
| Last Modified: | 13 Mar 2018 20:42 |
| PMCID: | PMC2278019 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/7722 |
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