Gesteland, R. F., Wolfner, M., Grisafi, P., Fink, G., Botstein, D., Roth, J. R. (March 1976) Yeast suppressors of UAA and UAG nonsense codons work efficiently in vitro via tRNA. Cell, 7 (3). pp. 381-90.
Abstract
A cell-free protein-synthesizing system, containing an S-100 fraction from yeast, ribosomal subunits from Krebs ascites cells, and ribosome initiation factors from rabbit reticulocytes, translates yeast, adenovirus, and rabbit globin messenger RNAs and the RNA from bacteriophage Qbeta.An amber mutation in the Qbeta synthetase gene is suppressed in vitro if the S-100 fraction s from yeast strains carrying amber suppressor mutations.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > analysis and processing > codon processing organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations |
| CSHL Authors: | |
| Communities: | Cold Spring Harbor Laboratory of Quantitative Biology |
| Depositing User: | Elizabeth Pessala |
| Date: | March 1976 |
| Date Deposited: | 25 Feb 2019 17:20 |
| Last Modified: | 25 Feb 2019 17:20 |
| URI: | https://repository.cshl.edu/id/eprint/37557 |
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