Rapid improvement of domestication traits in an orphan crop by genome editing

Lemmon, Z. H., Reem, N. T., Dalrymple, J., Soyk, S., Swartwood, K. E., Rodriguez-Leal, D., Van Eck, J., Lippman, Z. B. (October 2018) Rapid improvement of domestication traits in an orphan crop by genome editing. Nature Plants, 4 (10). pp. 766-770. ISSN 20550278 (ISSN)

URL: https://www.ncbi.nlm.nih.gov/pubmed/30287957
DOI: 10.1038/s41477-018-0259-x

Abstract

Genome editing holds great promise for increasing crop productivity, and there is particular interest in advancing breeding in orphan crops, which are often burdened by undesirable characteristics resembling wild relatives. We developed genomic resources and efficient transformation in the orphan Solanaceae crop 'groundcherry' (Physalis pruinosa) and used clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein-9 nuclease (Cas9) (CRISPR-Cas9) to mutate orthologues of tomato domestication and improvement genes that control plant architecture, flower production and fruit size, thereby improving these major productivity traits. Thus, translating knowledge from model crops enables rapid creation of targeted allelic diversity and novel breeding germplasm in distantly related orphan crops.

Item Type: Paper
Subjects: Investigative techniques and equipment > CRISPR-Cas9
organism description > plant > tomato
CSHL Authors:
Communities: CSHL labs > Lippman lab
Depositing User: Matthew Dunn
Date: 1 October 2018
Date Deposited: 01 Nov 2018 18:22
Last Modified: 01 Nov 2018 18:22
Related URLs:
URI: http://repository.cshl.edu/id/eprint/37284

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