A section-smear method for plant cytology

Warmke, Harry Earl (January 1941) A section-smear method for plant cytology. Stain Technology, 16 (1). pp. 9-12.

URL: http://www.tandfonline.com/doi/abs/10.3109/1052029...
DOI: 10.3109/10520294109106188

Abstract

An improved method for making root tip preparations of plants with medium or small chromosomes is described. Division figures are oriented in polar view by cutting paraffin cross sections of roots; the pectic substance is removed from the middle lamellae and the sections then are smeared. The procedure recommended is as follows: Kill root tips in Navashin or other fluid containing chromic acid. Wash, dehydrate, embed in paraffin, and section transversely. Stain slides bearing the sections by a Feulgen technic following the modification of De Tomasi, except that the hydrolysis is prolonged to 45 minutes, and a 10-minute washing in running water is introduced between the fuchsia and the first bath in sulfite solution.

Item Type: Paper
Subjects: Investigative techniques and equipment
organism description > plant
CSHL Authors:
Communities: The Carnegie Institution Department of Genetics
Depositing User: Elizabeth Pessala
Date: January 1941
Date Deposited: 26 Feb 2018 18:42
Last Modified: 24 Jul 2018 19:46
URI: http://repository.cshl.edu/id/eprint/36172

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