A retrovirus expressing the 12S adenoviral E1A gene product can immortalize epithelial cells from a broad range of rat tissues

Cone, R. D., Grodzicker, T., Jaramillo, M. (March 1988) A retrovirus expressing the 12S adenoviral E1A gene product can immortalize epithelial cells from a broad range of rat tissues. Molecular & Cellular Biology, 8 (3). pp. 1036-44. ISSN 0270-7306

URL: http://www.ncbi.nlm.nih.gov/pubmed/2966895
DOI: 10.1128/MCB.8.3.1036

Abstract

An epithelial cell-transforming virus could be of great use, both in the culture of epithelial cell lines and in the study of carcinogenesis. Since the adenoviral E1A gene has been shown to partially transform some epithelial cells from primary rat cell cultures, we constructed retrovirus vectors containing either the 12S or 13S E1A cDNA sequences to facilitate the transfer of these genes into a variety of primary cell types. The 12S E1A virus induced proliferation and immortalization of epithelial cells in rat kidney, liver, heart, pancreas, and thyroid primary cultures. In the two cases tested, heart and liver cultures, E1A-immortalized cells were nontumorigenic, but could be completely transformed by subsequent introduction of the ras oncogene. To our surprise, the 13S virus had a greatly reduced immortalization potential. We discuss these data in light of the model of Spindler et al. (K. R. Spindler, C. Y. Eng, and A.-J. Berk, J. Virol. 53:742-750, 1985), in which the 12S E1A protein is required for the complete induction of the cellular DNA replication machinery in the quiescent human epithelial cells in which adenoviruses normally replicate.

Item Type: Paper
Uncontrolled Keywords: Adenovirus Early Proteins Adenoviruses, Human/*genetics Animals Cell Division *Cell Transformation, Viral Cells, Cultured Epithelial Cells Fluorescent Antibody Technique *Genes, Viral Genetic Vectors Immunoassay Kidney/cytology Liver/cytology Myocardium/cytology Oncogene Proteins, Viral/*genetics Rats Rats, Inbred F344 Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Retroviridae/genetics
Subjects: organism description > virus > adenovirus
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cells

organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat

organism description > virus > retrovirus
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: March 1988
Date Deposited: 17 Oct 2017 17:33
Last Modified: 17 Oct 2017 17:33
PMCID: PMC363246
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35127

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