Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides

Hu, Q. J., Bautista, C., Edwards, G. M., Defeo-Jones, D., Jones, R. E., Harlow, E. (1991) Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides. Mol Cell Biol, 11 (11). pp. 5792-9. ISSN 0270-7306 (Print)0270-7306 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1717832
DOI: 10.1128/MCB.11.11.5792

Abstract

Even though the retinoblastoma gene is one of the best-studied tumor suppressor genes, little is known about its functional role. Like all tumor suppressor gene products, the retinoblastoma protein (pRB) is thought to inhibit some aspect of cell proliferation. It also appears to be a cellular target of several DNA tumor virus-transforming proteins, such as adenovirus E1A, human papillomavirus E7, or simian virus 40 large T antigen. To help in the analysis of pRB, we have prepared a new set of anti-human pRB monoclonal antibodies. In addition to being useful reagents for the study of human pRB, these antibodies display several unexpected properties. They can be used to distinguish different subsets of the pRBs on the basis of their phosphorylation states. Some are able to recognize pRB homologs in other species, including mice, chickens, and members of the genus Xenopus. In addition, some of these antibodies can bind directly to other cellular proteins that, like pRB, were originally identified through their association with adenovirus E1A. These immunologically cross-reactive proteins include the p107 and p300 proteins, and their recognition by antibodies raised against pRB suggests that several members of the E1A-targeted cellular proteins form a structurally and functionally related family.

Item Type: Paper
Uncontrolled Keywords: Adenovirus Early Proteins Animals *Antibodies, Monoclonal Antibody Specificity Antigens, Viral, Tumor/immunology Blotting, Western Chick Embryo Cross Reactions Epitopes/*analysis Genes, Retinoblastoma Humans Mice Oncogene Proteins, Viral/immunology Retinoblastoma Protein/analysis/genetics/*immunology
Subjects: organism description > virus > adenovirus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > tumor suppressor
CSHL Authors:
Communities: CSHL labs > Stillman lab
Depositing User: Matt Covey
Date Deposited: 14 Jan 2016 17:07
Last Modified: 14 Jan 2016 17:07
PMCID: PMC361950
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32041

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