Extracellular vesicle-mediated transfer of processed and functional RNY5 RNA

Chakrabortty, S. K., Prakash, A., Nechooshtan, G., Hearn, S., Gingeras, T. R. (November 2015) Extracellular vesicle-mediated transfer of processed and functional RNY5 RNA. RNA, 21 (11). pp. 1966-1979. ISSN 1469-9001 (Electronic)1355-8382 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/26392588
DOI: 10.1261/rna.053629.115

Abstract

Extracellular vesicles (EVs) have been proposed as a means to promote intercellular communication. We show that when human primary cells are exposed to cancer cell EVs, rapid cell death of the primary cells is observed, while cancer cells treated with primary or cancer cell EVs do not display this response. The active agents that trigger cell death are 29- to 31-nucleotide (nt) or 22- to 23-nt processed fragments of an 83-nt primary transcript of the human RNY5 gene that are highly likely to be formed within the EVs. Primary cells treated with either cancer cell EVs, deproteinized total RNA from either primary or cancer cell EVs, or synthetic versions of 31- and 23-nt fragments trigger rapid cell death in a dose-dependent manner. The transfer of processed RNY5 fragments through EVs may reflect a novel strategy used by cancer cells toward the establishment of a favorable microenvironment for their proliferation and invasion.

Item Type: Paper
Uncontrolled Keywords: Rny5 cancer microenvironment exosomes extracellular vesicles
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > Extracellular vesicles
CSHL Authors:
Communities: CSHL Cancer Center Program > Gene Regulation and Cell Proliferation
CSHL Cancer Center Shared Resources > Microscopy Service
CSHL labs > Gingeras lab
Depositing User: Matt Covey
Date: November 2015
Date Deposited: 01 Oct 2015 21:52
Last Modified: 09 Nov 2017 21:22
PMCID: PMC4604435
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31896

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