Chakrabortty, S. K., Prakash, A., Nechooshtan, G., Hearn, S., Gingeras, T. R. (November 2015) Extracellular vesicle-mediated transfer of processed and functional RNY5 RNA. RNA, 21 (11). pp. 1966-1979. ISSN 1469-9001 (Electronic)1355-8382 (Linking)
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Abstract
Extracellular vesicles (EVs) have been proposed as a means to promote intercellular communication. We show that when human primary cells are exposed to cancer cell EVs, rapid cell death of the primary cells is observed, while cancer cells treated with primary or cancer cell EVs do not display this response. The active agents that trigger cell death are 29- to 31-nucleotide (nt) or 22- to 23-nt processed fragments of an 83-nt primary transcript of the human RNY5 gene that are highly likely to be formed within the EVs. Primary cells treated with either cancer cell EVs, deproteinized total RNA from either primary or cancer cell EVs, or synthetic versions of 31- and 23-nt fragments trigger rapid cell death in a dose-dependent manner. The transfer of processed RNY5 fragments through EVs may reflect a novel strategy used by cancer cells toward the establishment of a favorable microenvironment for their proliferation and invasion.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Rny5 cancer microenvironment exosomes extracellular vesicles |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > Extracellular vesicles |
| CSHL Authors: | |
| Communities: | CSHL Cancer Center Program > Gene Regulation and Cell Proliferation CSHL Cancer Center Shared Resources > Microscopy Service CSHL labs > Gingeras lab CSHL Cancer Center Program > Cancer Genetics and Genomics Program |
| Depositing User: | Matt Covey |
| Date: | November 2015 |
| Date Deposited: | 01 Oct 2015 21:52 |
| Last Modified: | 05 Nov 2020 21:18 |
| PMCID: | PMC4604435 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31896 |
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