Expression cloning of a novel zinc finger protein that binds to the c-fos serum response element

Attar, R. M., Gilman, M. Z. (May 1992) Expression cloning of a novel zinc finger protein that binds to the c-fos serum response element. Mol Cell Biol, 12 (5). pp. 2432-43. ISSN 0270-7306 (Print)0270-7306 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1569959
DOI: 10.1128/MCB.12.5.2432

Abstract

Induction of c-fos transcription by serum growth factors requires the serum response element (SRE). The SRE is a multifunctional element which responds to several positively and negatively acting signals. To identify cellular proteins that might mediate functions of the SRE, we screened a human cDNA expression library with an SRE probe. We report the isolation and characterization of SRE-ZBP, a previously unidentified SRE-binding protein. SRE-ZBP is a member of the C2H2 zinc finger family of proteins exemplified by TFIIIA and the Drosophila Kruppel protein. The seven tandemly repeated zinc finger motifs in SRE-ZBP are sufficient for high-affinity binding to the SRE. We show that SRE-ZBP is a nuclear protein and identify a candidate cellular protein encoded by the SRE-ZBP gene. Because we cannot detect any DNA-binding activity attributable to the endogenous protein, we propose that SRE-ZBP activity may be subject to posttranslational regulation. Like c-fos mRNA, SRE-ZBP mRNA is serum inducible in HeLa cells, but with slower kinetics. The role of SRE-ZBP in the regulation of c-fos transcription remains unestablished, but this protein binds to a region of the SRE where mutations lead to derepression.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Base Sequence Cloning, Molecular/methods DNA/genetics/isolation & purification DNA Probes DNA-Binding Proteins/*metabolism Escherichia coli/genetics Fluorescent Antibody Technique Gene Library *Genes, fos Humans Immune Sera Molecular Sequence Data Nuclear Proteins/*metabolism Oligodeoxyribonucleotides Plasmids Recombinant Fusion Proteins/metabolism Sequence Homology, Nucleic Acid Serum Response Factor Substrate Specificity Transcription Factors/*metabolism Transfection Zinc Fingers/*genetics/physiology beta-Galactosidase/genetics/metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > c-fos
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > zinc finger proteins
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: May 1992
Date Deposited: 29 Sep 2015 15:46
Last Modified: 29 Sep 2015 15:46
PMCID: PMC364416
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31820

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