Telomerase activity in germline and embryonic cells of Xenopus

Mantell, L. L., Greider, C. W. (July 1994) Telomerase activity in germline and embryonic cells of Xenopus. Embo J, 13 (13). pp. 3211-7. ISSN 0261-4189 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8039513

Abstract

Telomerase is a ribonucleoprotein which synthesizes telomere repeats onto chromosome ends. Telomerase activity is involved in telomere length maintenance. We used Xenopus laevis as a model system to study the expression of telomerase activity in germline cells and during early development. We identified a non-processive telomerase activity in manually dissected nuclei of Xenopus stage VI oocytes. Telomerase activity was detected throughout oogenesis and embryogenesis. Telomerase was active in both S and M phase cell cycle extracts, suggesting that telomerase activity is not regulated with chromosomal DNA replication.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Cell Nucleus/enzymology DNA Nucleotidylexotransferase/ metabolism DNA Primers Female Male Mitosis Molecular Sequence Data Oogenesis Ovum/ enzymology Research Support, U.S. Gov't, P.H.S. S Phase Spermatozoa/ enzymology Telomere Tetrahymena Xenopus laevis
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > telomerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > telomeres
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Greider lab
Depositing User: Matt Covey
Date: 1 July 1994
Date Deposited: 03 Aug 2015 19:49
Last Modified: 03 Aug 2015 19:49
PMCID: PMC395213
URI: http://repository.cshl.edu/id/eprint/31444

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