Mantell, L. L., Greider, C. W. (July 1994) Telomerase activity in germline and embryonic cells of Xenopus. Embo J, 13 (13). pp. 3211-7. ISSN 0261-4189 (Print)
Abstract
Telomerase is a ribonucleoprotein which synthesizes telomere repeats onto chromosome ends. Telomerase activity is involved in telomere length maintenance. We used Xenopus laevis as a model system to study the expression of telomerase activity in germline cells and during early development. We identified a non-processive telomerase activity in manually dissected nuclei of Xenopus stage VI oocytes. Telomerase activity was detected throughout oogenesis and embryogenesis. Telomerase was active in both S and M phase cell cycle extracts, suggesting that telomerase activity is not regulated with chromosomal DNA replication.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Base Sequence Cell Nucleus/enzymology DNA Nucleotidylexotransferase/ metabolism DNA Primers Female Male Mitosis Molecular Sequence Data Oogenesis Ovum/ enzymology Research Support, U.S. Gov't, P.H.S. S Phase Spermatozoa/ enzymology Telomere Tetrahymena Xenopus laevis |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > telomerase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > telomeres organism description > animal > Frog > xenopus |
| CSHL Authors: | |
| Communities: | CSHL labs > Greider lab |
| Depositing User: | Matt Covey |
| Date: | 1 July 1994 |
| Date Deposited: | 03 Aug 2015 19:49 |
| Last Modified: | 03 Aug 2015 19:49 |
| PMCID: | PMC395213 |
| URI: | https://repository.cshl.edu/id/eprint/31444 |
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