Multiple regions of TBP participate in the response to transcriptional activators in vivo

Tansey, William P., Ruppert, Siegfried, Tjian, Robert, Herr, Winship (November 1994) Multiple regions of TBP participate in the response to transcriptional activators in vivo. Genes Dev, 8 (22). pp. 2756-69. ISSN 0890-9369 (Print)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/7958931
DOI: 10.1101/gad.8.22.2756

Abstract

We used mutant yeast and human TBP molecules with an altered DNA-binding specificity to examine the role of TBP in transcriptional activation in vivo. We show that yeast TBP is functionally equivalent to human TBP for response to numerous transcriptional activators in human cells, including those that do not function in yeast. Despite the extensive conservation of TBP, its ability to respond to transcriptional activators in vivo is curiously resistant to clustered sets of alanine substitution mutations in different regions of the protein, including those that disrupt DNA binding and basal transcription in vitro. Combined sets of these mutations, however, can attenuate the in vivo activity of TBP and can differentially affect response to different activation domains. Although the activity of TBP mutants in vivo did not correlate with DNA binding or basal transcription in vitro, it did correlate with binding in vitro to the largest subunit of TFIID, hTAFII250. Together, these data suggest that TBP utilizes multiple interactions across its surface to respond to RNA polymerase II transcriptional activators in vivo; some of these interactions appear to involve recruitment of TBP into TFIID, whereas others are involved in response to specific types of transcriptional activators.

Item Type: Paper
Uncontrolled Keywords: Gene Expression Regulation Humans Models, Molecular Mutation RNA Polymerase II/ biosynthesis Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. TATA Box Transcription Factor TFIID Transcription Factors/ genetics Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
CSHL Authors:
Communities: CSHL labs > Herr lab
CSHL labs > Tansey lab
Depositing User: Matt Covey
Date: 15 November 1994
Date Deposited: 25 Aug 2015 18:17
Last Modified: 03 Nov 2017 20:24
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31426

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