Regulation of protein kinase CKII during the cell division cycle

Marshak, D. R., Russo, G. L. (1994) Regulation of protein kinase CKII during the cell division cycle. Cell Mol Biol Res, 40 (5-6). pp. 513-7. ISSN 0968-8773 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7735325

Abstract

Protein kinase CKII is a prevalent serine/threonine protein kinase whose structure is highly conserved among eukaryotic organisms. Its involvement in the eukaryotic cell division cycle has been implicated by genetic experiments in yeast, antisense DNA, and inhibitory antibody experiments in mammalian cells, changes in activity during growth stimulation experiments, and protection of cells from radiation damage to replicating DNA. In addition, the cdc2 protein kinase, which is central to cell division cycle control, serves as a substrate for CKII specifically during the G1 phase of human cells. In this report, extracts of HeLa cells were prepared using neutral, aqueous buffers at low ionic strength. The cells were enriched for specific stages of the cell division cycle by treatment with drugs or by centrifugal elutriation. The results indicate that CKII activity in these extracts is highest during the G1 phase, and there appears to be a reduction in soluble CKII activity during the S phase. These data are consistent with the hypothesis that high CKII is necessary for a normal G1 phase but that progression through the S phase requires inhibition of CKII.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence CDC2 Protein Kinase/metabolism Casein Kinase II Cell Cycle Cell Division Cell Separation G1 Phase Gene Expression Regulation, Enzymologic Hela Cells/drug effects/enzymology Humans Hydroxyurea/pharmacology Molecular Sequence Data Neoplasm Proteins/metabolism Phosphorylation Protein Processing, Post-Translational Protein-Serine-Threonine Kinases/ metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell division
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date Deposited: 28 Aug 2015 14:42
Last Modified: 28 Aug 2015 14:42
URI: http://repository.cshl.edu/id/eprint/31401

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