Ballouz, S., Verleyen, W., Gillis, J. (February 2015) Guidance for RNA-seq co-expression network construction and analysis: safety in numbers. Bioinformatics. ISSN 1367-4803
Abstract
MOTIVATION: RNA-seq co-expression analysis is in its infancy and reasonable practices remain poorly defined. We assessed a variety of RNA-seq expression data to determine factors affecting functional connectivity and topology in co-expression networks. RESULTS: We examine RNA-seq co-expression data generated from 1,970 RNA-seq samples using a guilt-by-association framework, in which genes are assessed for the tendency of co-expression to reflect shared function. Minimal experimental criteria to obtain performance on par with microarrays were >20 samples with read depth >10M per sample. While the aggregate network constructed shows good performance (AUROC~0.71), the dependency on number of experiments used is nearly identical to that present in microarrays, suggesting thousands of samples to obtain 'gold-standard' co-expression. We find a major topological difference between RNA-seq and microarray co-expression in the form of low overlaps between hub-like genes from each network due to changes in the correlation of expression noise within each technology. Supplementary information: Networks are available at: http://gillislab.labsites.cshl.edu/supplements/rna-seq-networks/ and supplementary data are available at Bioinformatics online. CONTACT: jgillis@cshl.edu.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics bioinformatics > genomics and proteomics Investigative techniques and equipment > assays > RNA-seq |
| CSHL Authors: | |
| Communities: | CSHL labs > Gillis Lab Stanley Institute for Cognitive Genomics |
| Depositing User: | Matt Covey |
| Date: | 24 February 2015 |
| Date Deposited: | 06 Mar 2015 20:48 |
| Last Modified: | 06 Nov 2015 20:05 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31255 |
Actions (login required)
 |
Administrator's edit/view item |