Single-cell copy number analysis of prostate cancer cells captured with geometrically enhanced differential immunocapture microdevices

Pratt, E. D., Stepansky, A., Hicks, J., Kirby, B. J. (November 2014) Single-cell copy number analysis of prostate cancer cells captured with geometrically enhanced differential immunocapture microdevices. Analytical Chemistry, 86 (22). pp. 11013-7. ISSN 0003-2700

URL: http://www.ncbi.nlm.nih.gov/pubmed/25363873
DOI: 10.1021/ac503453v

Abstract

Limited access to tumor tissue makes repeated sampling and real-time tracking of cancer progression infeasible. Circulating tumor cells (CTCs) provide the capacity for real-time genetic characterization of a disseminating tumor cell population via a simple blood draw. However, there is no straightforward method to analyze broadscale genetic rearrangements in this heterogeneous cell population at the single cell level. We present a one-step controllable chemical extraction of whole nuclei from prostate cancer cells captured using geometrically enhanced differential immunocapture (GEDI) microdevices. We have successfully used copy number profile analysis to differentiate between two unique cancer cell line populations of metastatic origin (LNCaP and VCaP) and to analyze key mutations important in disease progression.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > copy number variants
diseases & disorders > cancer > cancer types > prostate cancer
CSHL Authors:
Communities: CSHL Cancer Center Program > Cancer Genetics
CSHL Cancer Center Shared Resources > Flow Cytometry Service
CSHL labs > Hicks lab
CSHL Cancer Center Shared Resources > DNA Sequencing Service
Depositing User: Matt Covey
Date: 18 November 2014
Date Deposited: 06 Jan 2015 15:11
Last Modified: 04 Nov 2015 21:25
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31011

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving