Expression of cell cycle proteins using recombinant baculoviruses

Hannon, G. J. (1995) Expression of cell cycle proteins using recombinant baculoviruses. In: Cell Cycle: Materials and Methods. Springer-Verlag, Berlin, pp. 231-242.

URL: http://link.springer.com/chapter/10.1007/978-3-642...
DOI: 10.1007/978-3-642-57783-3_20

Abstract

The ability to express proteins in heterologous systems has been crucial to the biochemical analysis of many cell cycle regulators. Until recently, bacterial expression systems have reigned supreme due to their ease of use and their ability to produce vast quantities of a desired protein. However, many proteins are recovered from bacteria in an inactive form due either to improper folding or to lack of critical covalent modifications (e.g., phosphorylation or glycosylation).

Item Type: Book Section
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression
organism description > virus
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Jessica Koos
Date: 1995
Date Deposited: 11 Aug 2014 16:18
Last Modified: 30 Nov 2016 21:37
URI: http://repository.cshl.edu/id/eprint/30599

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