Identification and Analysis of the Transcriptional Regulatory Networks Governing Mechanosensitive Channels in E. coli

Barnes, S., Jones, D., Belliveau, N., Sundaresh, S., Kinney, J., Phillips, R. (January 2014) Identification and Analysis of the Transcriptional Regulatory Networks Governing Mechanosensitive Channels in E. coli. Biophysical Journal, 106 (2; Sup). 487A-487A. ISSN 0006-34951542-0086

URL: http://www.sciencedirect.com/science/article/pii/S...
DOI: 10.1016/j.bpj.2013.11.4472

Abstract

Mechanosensitive (MS) channels are membrane proteins which are gated by mechanical stress in the cell membrane. Channel opening in response to mechanical stresses makes it possible for molecules to pass through the membrane, allowing an organism to alleviate solute imbalances which place osmotic stress on the membrane and can cause cell lysis and death. Two of the seven known MS channels in E. coli, MscL and MscS, are individually capable of rescuing these bacteria from sudden changes in osmolarity (osmotic shock). If MscL and MscS are both sufficient for ensuring survival under osmotic shock conditions, what is the purpose of the other five MS channels? In the interest of exploring the physiological roles of the various MS channels, we hypothesize that understanding how they are regulated will shed light on their function. Specifically, we investigate the regulatory context of the MS genes by identifying the regulatory architecture and transcription factors responsible for controlling MS gene output. We use a method known as "Sort-Seq" to locate transcription factor binding sites with base pair resolution, and DNA affinity chromatography to determine transcription factor identity. Understanding how the MS genes are regulated will give us clues as to the specific stressors which they defend the cell against. This work provides a specific case study of a method which we think will have much broader reach in mapping out regulatory networks genome-wide.

Item Type: Paper
Additional Information: Meeting Abstract
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
organism description > bacteria > escherichia coli
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
CSHL Authors:
Communities: CSHL labs > Kinney lab
Depositing User: Matt Covey
Date: 28 January 2014
Date Deposited: 11 Jul 2014 14:44
Last Modified: 11 Jul 2014 14:44
URI: http://repository.cshl.edu/id/eprint/30507

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