The mouse telomerase RNA 5"-end lies just upstream of the telomerase template sequence

Hinkley, C. S., Blasco, M. A., Funk, W. D., Feng, J., Villeponteau, B., Greider, C. W., Herr, W. (January 1998) The mouse telomerase RNA 5"-end lies just upstream of the telomerase template sequence. Nucleic Acids Research, 26 (2). pp. 532-6. ISSN 0305-1048

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URL: http://www.ncbi.nlm.nih.gov/pubmed/9421511
DOI: 10.1093/nar/26.2.532

Abstract

Telomerase is a ribonucleoprotein enzyme with an essential RNA component. Embedded within the telomerase RNA is a template sequence for telomere synthesis. We have characterized the structure of the 5' regions of the human and mouse telomerase-RNA genes, and have found a striking difference in the location of the template sequence: Whereas the 5'-end of the human telomerase RNA lies 45 nt from the telomerase-RNA template sequence, the 5'-end of the mouse telomerase RNA lies just 2 nt from the telomerase-RNA template sequence. Analysis of genomic sequences flanking the 5'-end of the human and mouse telomerase RNA-coding sequences reveals similar promoter-element arrangements typical of mRNA-type promoters: a TATA box-like element and an upstream region containing a consensus CCAAT box. This putative promoter structure contrasts with that of the ciliate telomerase-RNA genes whose structure resembles RNA polymerase III U6 small nuclear RNA (snRNA) promoters. These and other comparisons suggest that, during evolution, both the RNA-polymerase specificity of telomerase RNA-gene promoters and, more recently, the position of the template sequence in the telomerase RNA changed.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Consensus Sequence Humans Mice Molecular Sequence Data Promoter Regions (Genetics) RNA/ chemistry RNA, Messenger/chemistry Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Sequence Alignment TATA Box Telomerase/ chemistry Templates, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > alignment > sequence alignment
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > telomerase
CSHL Authors:
Communities: CSHL labs > Herr lab
Dolan DNA Learning Center
Depositing User: Kathleen Darby
Date: 15 January 1998
Date Deposited: 01 May 2014 18:31
Last Modified: 07 May 2014 13:16
PMCID: PMC147299
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29897

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