Hinkley, C. S., Blasco, M. A., Funk, W. D., Feng, J., Villeponteau, B., Greider, C. W., Herr, W. (January 1998) The mouse telomerase RNA 5"-end lies just upstream of the telomerase template sequence. Nucleic Acids Research, 26 (2). pp. 532-6. ISSN 0305-1048
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Abstract
Telomerase is a ribonucleoprotein enzyme with an essential RNA component. Embedded within the telomerase RNA is a template sequence for telomere synthesis. We have characterized the structure of the 5' regions of the human and mouse telomerase-RNA genes, and have found a striking difference in the location of the template sequence: Whereas the 5'-end of the human telomerase RNA lies 45 nt from the telomerase-RNA template sequence, the 5'-end of the mouse telomerase RNA lies just 2 nt from the telomerase-RNA template sequence. Analysis of genomic sequences flanking the 5'-end of the human and mouse telomerase RNA-coding sequences reveals similar promoter-element arrangements typical of mRNA-type promoters: a TATA box-like element and an upstream region containing a consensus CCAAT box. This putative promoter structure contrasts with that of the ciliate telomerase-RNA genes whose structure resembles RNA polymerase III U6 small nuclear RNA (snRNA) promoters. These and other comparisons suggest that, during evolution, both the RNA-polymerase specificity of telomerase RNA-gene promoters and, more recently, the position of the template sequence in the telomerase RNA changed.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Base Sequence Consensus Sequence Humans Mice Molecular Sequence Data Promoter Regions (Genetics) RNA/ chemistry RNA, Messenger/chemistry Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Sequence Alignment TATA Box Telomerase/ chemistry Templates, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > alignment > sequence alignment bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > telomerase |
| CSHL Authors: | |
| Communities: | CSHL labs > Herr lab Dolan DNA Learning Center |
| Depositing User: | Kathleen Darby |
| Date: | 15 January 1998 |
| Date Deposited: | 01 May 2014 18:31 |
| Last Modified: | 07 May 2014 13:16 |
| PMCID: | PMC147299 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29897 |
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