Autophosphorylation at Thr286 of the alpha calcium-calmodulin kinase II in LTP and learning

Giese, K. P., Fedorov, N. B., Filipkowski, R. K., Silva, A. J. (February 1998) Autophosphorylation at Thr286 of the alpha calcium-calmodulin kinase II in LTP and learning. Science, 279 (5352). pp. 870-3. ISSN 0036-8075 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9452388
DOI: 10.1126/science.279.5352.870

Abstract

The calcium-calmodulin-dependent kinase II (CaMKII) is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, CaMKII can undergo autophosphorylation, resulting in CaM-independent activity. A point mutation was introduced into the alphaCaMKII gene that blocked the autophosphorylation of threonine at position 286 (Thr286) of this kinase without affecting its CaM-dependent activity. The mutant mice had no N-methyl-D-aspartate receptor-dependent LTP in the hippocampal CA1 area and showed no spatial learning in the Morris water maze. Thus, the autophosphorylation of alphaCaMKII at Thr286 appears to be required for LTP and learning.

Item Type: Paper
Uncontrolled Keywords: 2-Amino-5-phosphonovalerate/pharmacology 6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology Animals Ca(2+)-Calmodulin Dependent Protein Kinase/genetics/ metabolism Calmodulin/metabolism Gene Targeting Hippocampus/metabolism/ physiology Long-Term Potentiation/drug effects Maze Learning Mice Mice, Inbred C57BL Mice, Mutant Strains Patch-Clamp Techniques Phosphorylation Phosphothreonine/metabolism Picrotoxin/pharmacology Point Mutation Pyramidal Cells/ physiology Receptors, N-Methyl-D-Aspartate/physiology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Synaptic Transmission
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > Long term potentiation
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
Investigative techniques and equipment > patch-clamp recording
Investigative techniques and equipment > recording devices > patch-clamp recording

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic transmission
CSHL Authors:
Communities: CSHL labs > Yin lab
Depositing User: Kathleen Darby
Date: 6 February 1998
Date Deposited: 01 May 2014 19:31
Last Modified: 01 May 2014 19:31
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29882

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