Giese, K. P., Fedorov, N. B., Filipkowski, R. K., Silva, A. J. (February 1998) Autophosphorylation at Thr286 of the alpha calcium-calmodulin kinase II in LTP and learning. Science, 279 (5352). pp. 870-3. ISSN 0036-8075 (Print)
Abstract
The calcium-calmodulin-dependent kinase II (CaMKII) is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, CaMKII can undergo autophosphorylation, resulting in CaM-independent activity. A point mutation was introduced into the alphaCaMKII gene that blocked the autophosphorylation of threonine at position 286 (Thr286) of this kinase without affecting its CaM-dependent activity. The mutant mice had no N-methyl-D-aspartate receptor-dependent LTP in the hippocampal CA1 area and showed no spatial learning in the Morris water maze. Thus, the autophosphorylation of alphaCaMKII at Thr286 appears to be required for LTP and learning.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | 2-Amino-5-phosphonovalerate/pharmacology 6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology Animals Ca(2+)-Calmodulin Dependent Protein Kinase/genetics/ metabolism Calmodulin/metabolism Gene Targeting Hippocampus/metabolism/ physiology Long-Term Potentiation/drug effects Maze Learning Mice Mice, Inbred C57BL Mice, Mutant Strains Patch-Clamp Techniques Phosphorylation Phosphothreonine/metabolism Picrotoxin/pharmacology Point Mutation Pyramidal Cells/ physiology Receptors, N-Methyl-D-Aspartate/physiology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Synaptic Transmission |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > Long term potentiation organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus Investigative techniques and equipment > patch-clamp recording Investigative techniques and equipment > recording devices > patch-clamp recording bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic transmission |
| CSHL Authors: | |
| Communities: | CSHL labs > Yin lab |
| Depositing User: | Kathleen Darby |
| Date: | 6 February 1998 |
| Date Deposited: | 01 May 2014 19:31 |
| Last Modified: | 01 May 2014 19:31 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29882 |
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