Integrated cytogenetic map of chromosome arm 4S of A-thaliana: Structural organization of heterochromatic knob and centromere region

Fransz, P. F., Armstrong, S., de Jong, J. H., Parnell, L. D., van Drunen, G., Dean, C., Zabel, P., Bisseling, T., Jones, G. H. (February 2000) Integrated cytogenetic map of chromosome arm 4S of A-thaliana: Structural organization of heterochromatic knob and centromere region. Cell, 100 (3). pp. 367-376. ISSN 0092-8674

URL: http://www.ncbi.nlm.nih.gov/pubmed/10676818
DOI: 10.1016/S0092-8674(00)80672-8

Abstract

We have constructed an integrated cytogenetic map of chromosome arm 4S of Arabidopsis thaliana. The map shows the detailed positions of various multicopy and unique sequences relative to euchromatin and heterochromatin segments. A quantitative analysis of the map positions at subsequent meiotic stages revealed a striking pattern of spatial and temporal variation in chromatin condensation for euchromatin and heterochromatin. For example, the centromere region consists of three domains with distinguishable structural, molecular, and functional properties. We also characterized a conspicuous heterochromatic knob of approximately 700 kb that accommodates a tandem repeat and several dispersed pericentromere-specific repeats. Moreover, our data provide evidence for an inversion event that relocated pericentromeric sequences to an interstitial position, resulting in the heterochromatic knob.

Item Type: Paper
Uncontrolled Keywords: RECOMBINANT INBRED LINES ARABIDOPSIS-THALIANA REPETITIVE DNA SEQUENCES GENOME RETROELEMENT LIBRARY MEIOSIS MARKERS ATHILA
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome > centromere
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome > centromere

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
CSHL Authors:
Communities: CSHL labs > McCombie lab
Depositing User: Matt Covey
Date: February 2000
Date Deposited: 30 Jan 2014 16:40
Last Modified: 30 Jan 2014 16:40
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29406

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