Interaction between the Drosophila CAF-1 and ASF1 chromatin assembly factors

Tyler, J. K., Collins, K. A., Prasad-Sinha, J., Amiott, E., Bulger, M., Harte, P. J., Kobayashi, R., Kadonaga, J. T. (October 2001) Interaction between the Drosophila CAF-1 and ASF1 chromatin assembly factors. Molecular and Cellular Biology, 21 (19). pp. 6574-6584. ISSN 0270-7306

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URL: http://www.ncbi.nlm.nih.gov/pubmed/11533245
DOI: 10.1128/MCB.21.19.6574-6584.2001

Abstract

The assembly of newly synthesized DNA into chromatin is essential for normal growth, development, and differentiation. To gain a better understanding of the assembly of chromatin during DNA synthesis, we identified, cloned, and characterized the 180- and 105-kDa polypeptides of Drosophila chromatin assembly factor 1 (dCAF-1). The purified recombinant p180+p105+p55 dCAF-1 complex is active for DNA replication-coupled chromatin assembly. Furthermore, we have established that the putative 75-kDa polypeptide of dCAF-1 is a C-terminally truncated form of p105 that does not coexist in dCAF-1 complexes containing the p105 subunit. The analysis of native and recombinant dCAF-1 revealed an interaction between dCAF-1 and the Drosophila anti-silencing function 1 (dASF1) component of replication-coupling assembly factor (RCAF). The binding of dASF1 to dCAF-1 is mediated through the p105 subunit of dCAF-1. Consistent with the interaction between dCAF-1 p105 and dASF1 in vitro, we observed that dASF1 and dCAF-1 p105 colocalized in vivo in Drosophila polytene chromosomes. This interaction between dCAF-1 and dASF1 may be a key component of the functional synergy observed between RCAF and dCAF-1 during the assembly of newly synthesized DNA into chromatin.

Item Type: Paper
Uncontrolled Keywords: DNA-REPLICATION INVITRO NEWLY SYNTHESIZED HISTONES FACTOR-I SACCHAROMYCES-CEREVISIAE WD REPEAT PROTEIN YEAST TRANSCRIPTION INHERITANCE COMPLEX
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > histone
CSHL Authors:
Communities: CSHL labs > Kobayashi lab
Depositing User: Matt Covey
Date: October 2001
Date Deposited: 16 Jan 2014 15:41
Last Modified: 16 Jan 2014 15:41
PMCID: PMC99803
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29321

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