Tyler, J. K., Collins, K. A., Prasad-Sinha, J., Amiott, E., Bulger, M., Harte, P. J., Kobayashi, R., Kadonaga, J. T. (October 2001) Interaction between the Drosophila CAF-1 and ASF1 chromatin assembly factors. Molecular and Cellular Biology, 21 (19). pp. 6574-6584. ISSN 0270-7306
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Abstract
The assembly of newly synthesized DNA into chromatin is essential for normal growth, development, and differentiation. To gain a better understanding of the assembly of chromatin during DNA synthesis, we identified, cloned, and characterized the 180- and 105-kDa polypeptides of Drosophila chromatin assembly factor 1 (dCAF-1). The purified recombinant p180+p105+p55 dCAF-1 complex is active for DNA replication-coupled chromatin assembly. Furthermore, we have established that the putative 75-kDa polypeptide of dCAF-1 is a C-terminally truncated form of p105 that does not coexist in dCAF-1 complexes containing the p105 subunit. The analysis of native and recombinant dCAF-1 revealed an interaction between dCAF-1 and the Drosophila anti-silencing function 1 (dASF1) component of replication-coupling assembly factor (RCAF). The binding of dASF1 to dCAF-1 is mediated through the p105 subunit of dCAF-1. Consistent with the interaction between dCAF-1 p105 and dASF1 in vitro, we observed that dASF1 and dCAF-1 p105 colocalized in vivo in Drosophila polytene chromosomes. This interaction between dCAF-1 and dASF1 may be a key component of the functional synergy observed between RCAF and dCAF-1 during the assembly of newly synthesized DNA into chromatin.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | DNA-REPLICATION INVITRO NEWLY SYNTHESIZED HISTONES FACTOR-I SACCHAROMYCES-CEREVISIAE WD REPEAT PROTEIN YEAST TRANSCRIPTION INHERITANCE COMPLEX |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification organism description > animal > insect > Drosophila bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > histone |
| CSHL Authors: | |
| Communities: | CSHL labs > Kobayashi lab |
| Depositing User: | Matt Covey |
| Date: | October 2001 |
| Date Deposited: | 16 Jan 2014 15:41 |
| Last Modified: | 16 Jan 2014 15:41 |
| PMCID: | PMC99803 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29321 |
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