Single-cell electroporation for gene transfer in vivo

Haas, K., Sin, W. C., Javaherian, A., Li, Z., Cline, H. T. (March 2001) Single-cell electroporation for gene transfer in vivo. Neuron, 29 (3). pp. 583-591. ISSN 0896-6273

URL: http://www.ncbi.nlm.nih.gov/pubmed/11301019
DOI: 10.1016/S0896-6273(01)00235-5

Abstract

We report an electroporation technique for targeting gene transfer to individual cells in intact tissue. Electrical stimulation through a micropipette filled with DNA or other macromolecules electroporates a single cell at the tip of the micropipette. Electroporation of a plasmid encoding enhanced green fluorescent protein (GFP) into the brain of intact Xenopus tadpoles or rat hippocampal slices resulted in GFP expression in single neurons and glia. In vivo imaging showed morphologies, dendritic arbor dynamics, and growth rates characteristic of healthy cells. Coelectroporation of two plasmids resulted in expression of both proteins, while electroporation of fluorescent dextrans allowed direct visualization of transfer of molecules into cells. This technique will allow unprecedented spatial and temporal control over gene delivery and protein expression.

Item Type: Paper
Uncontrolled Keywords: IN-VIVO ARBOR GROWTH EXPRESSION CAMKII RECEPTORS PROTEINS EMBRYOS NEURONS TISSUE OVO
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene transfer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
Investigative techniques and equipment > transfection > single cell electroporation
CSHL Authors:
Communities: CSHL labs > Cline lab
Depositing User: Matt Covey
Date: March 2001
Date Deposited: 22 Jan 2014 19:16
Last Modified: 22 Jan 2014 19:16
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29240

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