Evidence that Candida stellatoidea type II is a mutant of Candida albicans that does not express sucrose-inhibitable α-glucosidase

Kwon-Chung, K. J., Hicks, J. B., Lipke, P. N. (1990) Evidence that Candida stellatoidea type II is a mutant of Candida albicans that does not express sucrose-inhibitable α-glucosidase. Infection and Immunity, 58 (9). pp. 2804-2808. ISSN 00199567 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1974885

Abstract

Candida stellatoidea is classically distinguished from C. albicans by the ability of the latter species to assimilate sucrose. We show here that sucrose-positive revertants of C. stellatoidea type II are readily isolated and that C. stellatoidea type II strains probably resulted from a mutation in the sucrase gene of C. albicans. The revertants were not laboratory contaminants, as determined by restriction fragment length polymorphism analysis and retention of an auxotrophic marker. The reversion of three tested strains was accompanied by 16- to 110-fold increases in expression of a sucrase/α-glucosidase but not an invertase, with a K(m) for sucrose of about 1 mM. The enzyme activity was assayable in intact cells. The drastically increased expression of such an enzyme would allow extracellular sucrose hydrolysis and assimilation of the monosaccharide products.

Item Type: Paper
Uncontrolled Keywords: alpha glucosidase article candida albicans fungus nonhuman priority journal yeast mutant alpha-Glucosidases Animal Candida Female Genetic Markers Kinetics Mice Mice, Inbred BALB C Mutation Phenotype Polymorphism, Restriction Fragment Length Sucrase Sucrose Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. Virulence
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Hicks lab
Depositing User: Matt Covey
Date: 1990
Date Deposited: 09 Aug 2013 20:55
Last Modified: 12 Aug 2013 14:57
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28498

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