Kwon-Chung, K. J., Hicks, J. B., Lipke, P. N. (1990) Evidence that Candida stellatoidea type II is a mutant of Candida albicans that does not express sucrose-inhibitable α-glucosidase. Infection and Immunity, 58 (9). pp. 2804-2808. ISSN 00199567 (ISSN)
Abstract
Candida stellatoidea is classically distinguished from C. albicans by the ability of the latter species to assimilate sucrose. We show here that sucrose-positive revertants of C. stellatoidea type II are readily isolated and that C. stellatoidea type II strains probably resulted from a mutation in the sucrase gene of C. albicans. The revertants were not laboratory contaminants, as determined by restriction fragment length polymorphism analysis and retention of an auxotrophic marker. The reversion of three tested strains was accompanied by 16- to 110-fold increases in expression of a sucrase/α-glucosidase but not an invertase, with a K(m) for sucrose of about 1 mM. The enzyme activity was assayable in intact cells. The drastically increased expression of such an enzyme would allow extracellular sucrose hydrolysis and assimilation of the monosaccharide products.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | alpha glucosidase article candida albicans fungus nonhuman priority journal yeast mutant alpha-Glucosidases Animal Candida Female Genetic Markers Kinetics Mice Mice, Inbred BALB C Mutation Phenotype Polymorphism, Restriction Fragment Length Sucrase Sucrose Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. Virulence |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Hicks lab |
| Depositing User: | Matt Covey |
| Date: | 1990 |
| Date Deposited: | 09 Aug 2013 20:55 |
| Last Modified: | 12 Aug 2013 14:57 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28498 |
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