a/α-Specific repression by MATα2

Strathern, J., Shafer, B., Hicks, J., McGill, C. (1988) a/α-Specific repression by MATα2. Genetics, 120 (1). pp. 75-81. ISSN 00166731 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2851482

Abstract

The product of the MAT alpha 2 gene is a DNA-binding protein that acts as a repressor of two different sets of cell type-specific genes. In alpha cells, the alpha 2 protein represses the transcription of several a-specific genes. In a/alpha cells, the alpha 2 protein acts together with the product of the MATa1 gene, the a1 protein, to repress several genes used by haploids in the mating process. In addition to the mat alpha 2 mutations that result in defects in both types of regulation, other mat alpha 2 alleles have been described that result in defects in the repression of a-specific genes but that do not affect the ability of the alpha 2 and a1 proteins to interact to repress the haploid-specific genes. We report here the isolation of a new class of mat alpha 2 mutations that do not affect the ability of the alpha 2 protein to repress a-specific genes, but that interfere with the ability of the alpha 2 protein to interact with the a1 protein to repress the haploid-specific genes and establish the a/alpha cell type. These mutations may help determine the means by which the a1 protein interacts with alpha 2 to expand the set of genes under its control.

Item Type: Paper
Uncontrolled Keywords: dna binding protein fungus gene expression genetic engineering nonhuman saccharomyces cerevisiae Amino Acid Sequence Base Sequence DNA Restriction Enzymes DNA-Binding Proteins Genes, Fungal Genes, Structural Molecular Sequence Data Mutation Plasmids Repressor Proteins Support, U.S. Gov't, P.H.S. Transcription Factors
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
CSHL Authors:
Communities: CSHL labs > Hicks lab
Depositing User: Matt Covey
Date: 1988
Date Deposited: 09 Aug 2013 20:55
Last Modified: 12 Aug 2013 15:02
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28497

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