Immunoblotting histones from yeast whole-cell protein extracts

Rossmann, M. P., Stillman, B. (2013) Immunoblotting histones from yeast whole-cell protein extracts. Cold Spring Harbor Protocols, 2013 (7). ISSN 1559-6095

URL: http://www.ncbi.nlm.nih.gov/pubmed/23818662
DOI: 10.1101/pdb.prot067116

Abstract

Histones are small basic proteins that are core components of chromatin. As such, they are essential for cell viability and genomic stability and their levels are tightly controlled. In addition, histone tails are subject to extensive posttranslational modifications, including acetylation, methylation, phosphorylation and ubiquitylation, that play critical roles in many cellular processes. To quickly screen for alterations in histone levels and/or their modifications in yeast mutants under different growth conditions, we present a fast and reliable protocol for whole-cell protein extract preparation and immunoblotting.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > histone
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: Matt Covey
Date Deposited: 19 Jul 2013 15:55
Last Modified: 20 Jun 2017 16:23
PMCID: PMC4085693
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28449

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