Immunoblotting histones from yeast whole-cell protein extracts

Rossmann, M. P., Stillman, B. (July 2013) Immunoblotting histones from yeast whole-cell protein extracts. Cold Spring Harbor Protocols, 2013 (7). ISSN 1559-6095

Abstract

Histones are small basic proteins that are core components of chromatin. As such, they are essential for cell viability and genomic stability and their levels are tightly controlled. In addition, histone tails are subject to extensive posttranslational modifications, including acetylation, methylation, phosphorylation and ubiquitylation, that play critical roles in many cellular processes. To quickly screen for alterations in histone levels and/or their modifications in yeast mutants under different growth conditions, we present a fast and reliable protocol for whole-cell protein extract preparation and immunoblotting.

Item Type:	Paper
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > histone bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types organism description > yeast
CSHL Authors:	Rossmann, Marlies P. Stillman, Bruce W.
Communities:	CSHL labs > Stillman lab
Highlight:	Stillman, Bruce W.
Depositing User:	Matt Covey
Date:	1 July 2013
Date Deposited:	19 Jul 2013 15:55
Last Modified:	20 Jun 2017 16:23
PMCID:	PMC4085693
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/28449

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