Cloning, localization, and permanent expression of a Drosophila octopamine receptor

Arakawa, S., Gocayne, J. D., McCombie, W. R., Urquhart, D. A., Hall, L. M., Fraser, C. M., Venter, J. C. (1990) Cloning, localization, and permanent expression of a Drosophila octopamine receptor. Neuron, 4 (3). pp. 343-354. ISSN 08966273 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2156539
DOI: 10.1016/0896-6273(90)90047-j

Abstract

A cDNA for a member of the G protein-coupled receptor family was isolated from Drosophila using a probe derived from a human β2-adrenergic receptor cDNA. This Drosophila receptor gene is localized at 99A10-B1 on the right arm of chromosome 3 and is preferentially expressed in Drosophila heads. The insect octopamine receptor has been permanently expressed in mammalian cells, where it mediates the attenuation of adenylate cyclase activity and exhibits a pharmacological profile consistent with an octopamine type 1 receptor. Sequence and pharmacological comparisons indicate that the octopamine receptor is unique but closely related to mammalian adrenergic receptors, perhaps as an evolutionary precursor.

Item Type: Paper
Uncontrolled Keywords: adenylate cyclase adrenergic receptor chlorpromazine clonidine neurotransmitter receptor octopamine oxedrine serotonin yohimbine animal cell arthropod article chromosome 3 drosophila evolution gene expression genetic engineering nonhuman priority journal Amino Acid Sequence Animal Base Sequence Brain Cell Line Chromosome Mapping Cloning, Molecular DNA Genes Genomic Library GTP-Binding Proteins Human Molecular Sequence Data Oligonucleotide Probes Protein Conformation Receptors, Adrenergic Receptors, Adrenergic, beta Restriction Mapping Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Transfection
Subjects: organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
Investigative techniques and equipment
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
Investigative techniques and equipment > assays
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

organism description > animal > insect
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein receptor
CSHL Authors:
Communities: CSHL labs > McCombie lab
Depositing User: Matt Covey
Date Deposited: 25 Apr 2013 16:57
Last Modified: 25 Apr 2013 16:57
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28199

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