Arakawa, S., Gocayne, J. D., McCombie, W. R., Urquhart, D. A., Hall, L. M., Fraser, C. M., Venter, J. C. (1990) Cloning, localization, and permanent expression of a Drosophila octopamine receptor. Neuron, 4 (3). pp. 343-354. ISSN 08966273 (ISSN)
Abstract
A cDNA for a member of the G protein-coupled receptor family was isolated from Drosophila using a probe derived from a human β2-adrenergic receptor cDNA. This Drosophila receptor gene is localized at 99A10-B1 on the right arm of chromosome 3 and is preferentially expressed in Drosophila heads. The insect octopamine receptor has been permanently expressed in mammalian cells, where it mediates the attenuation of adenylate cyclase activity and exhibits a pharmacological profile consistent with an octopamine type 1 receptor. Sequence and pharmacological comparisons indicate that the octopamine receptor is unique but closely related to mammalian adrenergic receptors, perhaps as an evolutionary precursor.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | adenylate cyclase adrenergic receptor chlorpromazine clonidine neurotransmitter receptor octopamine oxedrine serotonin yohimbine animal cell arthropod article chromosome 3 drosophila evolution gene expression genetic engineering nonhuman priority journal Amino Acid Sequence Animal Base Sequence Brain Cell Line Chromosome Mapping Cloning, Molecular DNA Genes Genomic Library GTP-Binding Proteins Human Molecular Sequence Data Oligonucleotide Probes Protein Conformation Receptors, Adrenergic Receptors, Adrenergic, beta Restriction Mapping Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Transfection |
| Subjects: | organism description > animal > insect > Drosophila bioinformatics > genomics and proteomics > genetics & nucleic acid processing Investigative techniques and equipment bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification Investigative techniques and equipment > assays Investigative techniques and equipment > cloning Investigative techniques and equipment > assays > cloning organism description > animal > insect bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein receptor |
| CSHL Authors: | |
| Communities: | CSHL labs > McCombie lab |
| Depositing User: | Matt Covey |
| Date: | 1990 |
| Date Deposited: | 25 Apr 2013 16:57 |
| Last Modified: | 25 Apr 2013 16:57 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28199 |
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