The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation

Diao, A., Rahman, D., Pappin, D. J., Lucocq, J., Lowe, M. (January 2003) The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation. Journal of Cell Biology, 160 (2). pp. 201-12. ISSN 0021-9525

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URL: http://www.ncbi.nlm.nih.gov/pubmed/12538640
DOI: 10.1083/jcb.200207045

Abstract

Fragmentation of the mammalian Golgi apparatus during mitosis requires the phosphorylation of a specific subset of Golgi-associated proteins. We have used a biochemical approach to characterize these proteins and report here the identification of golgin-84 as a novel mitotic target. Using cryoelectron microscopy we could localize golgin-84 to the cis-Golgi network and found that it is enriched on tubules emanating from the lateral edges of, and often connecting, Golgi stacks. Golgin-84 binds to active rab1 but not cis-Golgi matrix proteins. Overexpression or depletion of golgin-84 results in fragmentation of the Golgi ribbon. Strikingly, the Golgi ribbon is converted into mini-stacks constituting only approximately 25% of the volume of a normal Golgi apparatus upon golgin-84 depletion. These mini-stacks are able to carry out protein transport, though with reduced efficiency compared with a normal Golgi apparatus. Our results suggest that golgin-84 plays a key role in the assembly and maintenance of the Golgi ribbon in mammalian cells.

Item Type: Paper
Uncontrolled Keywords: Animals Autoantigens Endoplasmic Reticulum/metabolism/ultrastructure Eukaryotic Cells/ metabolism/ultrastructure Gene Expression Regulation/physiology Golgi Apparatus/ metabolism/ultrastructure HeLa Cells Humans Intracellular Membranes/ metabolism/ultrastructure Membrane Proteins/ metabolism/ultrastructure Microscopy, Electron Microtubule Proteins/ metabolism/ultrastructure Mitosis/genetics Phosphoproteins/metabolism Protein Transport/genetics RNA, Small Interfering/diagnostic use Rats Recombinant Fusion Proteins/diagnostic use Subcellular Fractions Viral Proteins/diagnostic use rab1 GTP-Binding Proteins/ metabolism/ultrastructure
Subjects: physics > biophysics > membrane dynamics
organs, tissues, organelles, cell types and functions > tissues types and functions > membranes
organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:
Communities: CSHL labs > Pappin lab
Depositing User: Matt Covey
Date: 20 January 2003
Date Deposited: 01 Jul 2013 19:47
Last Modified: 01 Jul 2013 19:47
PMCID: PMC2172652
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27876

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