Promoters and processing sites within the transforming region of bovine papillomavirus type 1

Ahola, H., Stenlund, A., Moreno-Lopez, J., Pettersson, U. (July 1987) Promoters and processing sites within the transforming region of bovine papillomavirus type 1. J Virol, 61 (7). pp. 2240-4. ISSN 0022-538X (Print)0022-538X (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2884331

Abstract

The mRNAs present in bovine papillomavirus type 1 (BPV-1)-transformed C127 cells were studied by primer extension. The results show that two internal promoters are present in the E region of BPV-1 in addition to the previously identified promoter at coordinate 1 (H. Ahola, A. Stenlund, J. Moreno-Lopez, and U. Pettersson, Nucleic Acids Res. 11:2639-2650, 1983). One, located at coordinate 31, generated a set of mRNAs with heterogeneous 5' ends, which may encode the major transforming protein of BPV-1, the E5 protein. The second promoter, which is located at coordinate 39, generates colinear mRNAs which encode either the E4 protein or a truncated form of the E2 protein. Unlike the cottontail rabbit papillomavirus (O. Danos, E. Georges, G. Orth, and M. Yaniv, J. Virol. 53:735-741, 1985), BPV-1 appears to lack a separate promoter for expression of the E7 protein. The major splice sites in the transforming region (E region) of the BPV-1 genome were also identified by nucleotide sequence analysis.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Bovine papillomavirus 1/ genetics DNA/genetics Gene Expression Regulation Papillomaviridae/ genetics Poly A/ genetics Promoter Regions, Genetic RNA Caps/genetics RNA, Messenger/ genetics RNA, Viral/ genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics
organism description > virus
CSHL Authors:
Communities: CSHL labs > Stenlund lab
Depositing User: Matt Covey
Date: July 1987
Date Deposited: 12 Mar 2013 18:35
Last Modified: 12 Mar 2013 18:35
PMCID: PMC283688
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27779

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