Ahola, H., Stenlund, A., Moreno-Lopez, J., Pettersson, U. (July 1987) Promoters and processing sites within the transforming region of bovine papillomavirus type 1. J Virol, 61 (7). pp. 2240-4. ISSN 0022-538X (Print)0022-538X (Linking)
Abstract
The mRNAs present in bovine papillomavirus type 1 (BPV-1)-transformed C127 cells were studied by primer extension. The results show that two internal promoters are present in the E region of BPV-1 in addition to the previously identified promoter at coordinate 1 (H. Ahola, A. Stenlund, J. Moreno-Lopez, and U. Pettersson, Nucleic Acids Res. 11:2639-2650, 1983). One, located at coordinate 31, generated a set of mRNAs with heterogeneous 5' ends, which may encode the major transforming protein of BPV-1, the E5 protein. The second promoter, which is located at coordinate 39, generates colinear mRNAs which encode either the E4 protein or a truncated form of the E2 protein. Unlike the cottontail rabbit papillomavirus (O. Danos, E. Georges, G. Orth, and M. Yaniv, J. Virol. 53:735-741, 1985), BPV-1 appears to lack a separate promoter for expression of the E7 protein. The major splice sites in the transforming region (E region) of the BPV-1 genome were also identified by nucleotide sequence analysis.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Sequence Bovine papillomavirus 1/ genetics DNA/genetics Gene Expression Regulation Papillomaviridae/ genetics Poly A/ genetics Promoter Regions, Genetic RNA Caps/genetics RNA, Messenger/ genetics RNA, Viral/ genetics |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs > Stenlund lab |
| Depositing User: | Matt Covey |
| Date: | July 1987 |
| Date Deposited: | 12 Mar 2013 18:35 |
| Last Modified: | 12 Mar 2013 18:35 |
| PMCID: | PMC283688 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27779 |
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