Circumventricular organs: A novel site of neural stem cells in the adult brain

Bennett, Lori, Yang, Ming, Enikolopov, G. N., Iacovitti, Lorraine (June 2009) Circumventricular organs: A novel site of neural stem cells in the adult brain. Molecular and Cellular Neuroscience, 41 (3). pp. 337-347.

URL: http://www.ncbi.nlm.nih.gov/pubmed/19409493
DOI: 10.1016/j.mcn.2009.04.007

Abstract

Neurogenesis in the adult mammalian nervous system is now well established in the subventricular zone of the anterolateral ventricle and subgranular zone of the hippocampus. In these regions, neurons are thought to arise from neural stem cells, identified by their expression of specific intermediate filament proteins (nestin, vimentin, GFAP) and transcription factors (Sox2). In the present study, we show that in adult rat and mouse, the circumventricular organs (CVOs) are rich in nestin+, GFAP+, vimentin+ cells which express Sox2 and the cell cycle-regulating protein Ki67. In culture, these cells proliferate as neurospheres and express neuronal (doublecortin+, [beta]-tubulin III+) and glial (S100[beta]+, GFAP+, RIP+) phenotypic traits. Further, our in vivo studies using bromodeoxyuridine show that CVO cells proliferate and undergo constitutive neurogenesis and gliogenesis. These findings suggest that CVOs may constitute a heretofore unknown source of stem/progenitor cells, capable of giving rise to new neurons and/or glia in the adult brain.

Item Type: Paper
Uncontrolled Keywords: Neural stem cell Neurogenesis Gliogenesis Nestin Sox2 Ki67 Vimentin
Subjects: organism description > animal > developmental stage > adult
organs, tissues, organelles, cell types and functions > organs types and functions > brain
organs, tissues, organelles, cell types and functions > organs types and functions
organs, tissues, organelles, cell types and functions
therapies > stem cells
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
CSHL Cancer Center Shared Resources > Animal Services
CSHL Cancer Center Shared Resources > Microscopy Service
Depositing User: Matt Covey
Date: 22 June 2009
Date Deposited: 21 Feb 2013 19:31
Last Modified: 30 Dec 2014 16:58
PMCID: PMC2697272
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27343

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving